Primer Details for TRINITY_DN21204_c0_g1_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTTTAAAAGGTGGGGCCC | ACTCTCTCAGCAACGTTAAATGT | Start: 52 |
| Tm | 59.312 | 58.368 | End: 226 |
| Size | 20 | 23 | 175 |
| Primer 2 details |
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| Sequence | GCAGGAAGCATGGATCGATG | ACTCTCTCAGCAACGTTAAATGT | Start: 0 |
| Tm | 59.127 | 58.368 | End: 226 |
| Size | 20 | 23 | 227 |
| Primer 3 details |
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| Sequence | GGTTTTAAAAGGTGGGGCCC | ACTCTCTCAGCAACGTTAAATGTTG | Start: 52 |
| Tm | 59.312 | 60.05 | End: 226 |
| Size | 20 | 25 | 175 |
| Primer 4 details |
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| Sequence | GGTTTTAAAAGGTGGGGCCC | CACATTTTAACTCTCTCAGCAACGT | Start: 52 |
| Tm | 59.312 | 60.05 | End: 235 |
| Size | 20 | 25 | 184 |
| Primer 5 details |
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| Sequence | GGTTTTAAAAGGTGGGGCCC | ACACATTTTAACTCTCTCAGCAACG | Start: 52 |
| Tm | 59.312 | 60.05 | End: 236 |
| Size | 20 | 25 | 185 |
