ssr_primer
Primer Details for TRINITY_DN51138_c0_g1_i2 |
|---|
| (G)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCGGTTTTTCGTCTGCGG | ATGCACTCCGTCAGCGTAAA | Start: 248 |
| Tm | 60.179 | 60.038 | End: 509 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
|---|
| Sequence | GGCCCTCCCCTTTATTGACC | CCGCAGACGAAAAACCGATG | Start: 67 |
| Tm | 60.106 | 60.179 | End: 267 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | ATCGGTTTTTCGTCTGCGGA | ATGCACTCCGTCAGCGTAAA | Start: 249 |
| Tm | 60.32 | 60.038 | End: 509 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | GGCCCTCCCCTTTATTGACC | TCCGCAGACGAAAAACCGAT | Start: 67 |
| Tm | 60.106 | 60.32 | End: 268 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | CATCGGTTTTTCGTCTGCGG | TGCACTCCGTCAGCGTAAAA | Start: 248 |
| Tm | 60.179 | 60.25 | End: 508 |
| Size | 20 | 20 | 261 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCGGTTTTTCGTCTGCGG | ATGCACTCCGTCAGCGTAAA | Start: 248 |
| Tm | 60.179 | 60.038 | End: 509 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
|---|
| Sequence | GGCCCTCCCCTTTATTGACC | CCGCAGACGAAAAACCGATG | Start: 67 |
| Tm | 60.106 | 60.179 | End: 267 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | ATCGGTTTTTCGTCTGCGGA | ATGCACTCCGTCAGCGTAAA | Start: 249 |
| Tm | 60.32 | 60.038 | End: 509 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | GGCCCTCCCCTTTATTGACC | TCCGCAGACGAAAAACCGAT | Start: 67 |
| Tm | 60.106 | 60.32 | End: 268 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | CATCGGTTTTTCGTCTGCGG | TGCACTCCGTCAGCGTAAAA | Start: 248 |
| Tm | 60.179 | 60.25 | End: 508 |
| Size | 20 | 20 | 261 |
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