ssr_primer
Primer Details for TRINITY_DN55569_c0_g1_i1 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATTGCGGCGTTCTCCTCTC | ATAGTGCGATCAGACACCGG | Start: 221 |
| Tm | 59.898 | 59.614 | End: 363 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CCGGTGTCTGATCGCACTAT | GAGAGGAGAACGCCGCAATA | Start: 41 |
| Tm | 59.614 | 59.898 | End: 240 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | CGGCGTTCTCCTCTCACAAT | ATAGTGCGATCAGACACCGG | Start: 226 |
| Tm | 60.109 | 59.614 | End: 363 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | CCGGTGTCTGATCGCACTAT | ATTGTGAGAGGAGAACGCCG | Start: 41 |
| Tm | 59.614 | 60.109 | End: 245 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | TATTGCGGCGTTCTCCTCTC | GACACCGGTCTATTCGCAGG | Start: 221 |
| Tm | 59.898 | 60.528 | End: 351 |
| Size | 20 | 20 | 131 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATTGCGGCGTTCTCCTCTC | ATAGTGCGATCAGACACCGG | Start: 221 |
| Tm | 59.898 | 59.614 | End: 363 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CCGGTGTCTGATCGCACTAT | GAGAGGAGAACGCCGCAATA | Start: 41 |
| Tm | 59.614 | 59.898 | End: 240 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | CGGCGTTCTCCTCTCACAAT | ATAGTGCGATCAGACACCGG | Start: 226 |
| Tm | 60.109 | 59.614 | End: 363 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | CCGGTGTCTGATCGCACTAT | ATTGTGAGAGGAGAACGCCG | Start: 41 |
| Tm | 59.614 | 60.109 | End: 245 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | TATTGCGGCGTTCTCCTCTC | GACACCGGTCTATTCGCAGG | Start: 221 |
| Tm | 59.898 | 60.528 | End: 351 |
| Size | 20 | 20 | 131 |
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