Primer Details for TRINITY_DN56386_c0_g1_i1 |
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| (A)19cc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTTATCAATCTCTGTTTGATCCG | TCCCTGAGAAAGTACCTCAACA | Start: 248 |
| Tm | 59.183 | 58.417 | End: 378 |
| Size | 25 | 22 | 131 |
| Primer 2 details |
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| Sequence | GGTTATCAATCTCTGTTTGATCCGA | TCCCTGAGAAAGTACCTCAACA | Start: 249 |
| Tm | 58.951 | 58.417 | End: 378 |
| Size | 25 | 22 | 130 |
| Primer 3 details |
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| Sequence | TGGTTATCAATCTCTGTTTGATCCG | TTCCCTGAGAAAGTACCTCAACA | Start: 248 |
| Tm | 59.183 | 59.029 | End: 379 |
| Size | 25 | 23 | 132 |
| Primer 4 details |
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| Sequence | TGGTTATCAATCTCTGTTTGATCCG | TCCCTGAGAAAGTACCTCAACATG | Start: 248 |
| Tm | 59.183 | 60.02 | End: 378 |
| Size | 25 | 24 | 131 |
| Primer 5 details |
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| Sequence | GGTTATCAATCTCTGTTTGATCCG | TCCCTGAGAAAGTACCTCAACA | Start: 249 |
| Tm | 57.708 | 58.417 | End: 378 |
| Size | 24 | 22 | 130 |
