ssr_primer
Primer Details for TRINITY_DN62002_c0_g1_i2 |
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| (T)58: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCGAGGTGTCGGTGGAAT | CGATACGGGATGGGAATGGG | Start: 50 |
| Tm | 60.038 | 60.036 | End: 294 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
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| Sequence | CGAGGTGTCGGTGGAATGAA | CGATACGGGATGGGAATGGG | Start: 53 |
| Tm | 60.038 | 60.036 | End: 294 |
| Size | 20 | 20 | 242 |
| Primer 3 details |
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| Sequence | TTCGAGGTGTCGGTGGAATG | CGATACGGGATGGGAATGGG | Start: 51 |
| Tm | 60.038 | 60.036 | End: 294 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
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| Sequence | GAACGCACGAGTTGGAATCG | CGATACGGGATGGGAATGGG | Start: 70 |
| Tm | 59.907 | 60.036 | End: 294 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
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| Sequence | CATCAAAGCGAGCGCATCAA | CCAAGTCGTTCGAAGGGGAA | Start: 780 |
| Tm | 59.903 | 59.966 | End: 960 |
| Size | 20 | 20 | 181 |
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