ssr_primer
Primer Details for TRINITY_DN63591_c2_g1_i2 |
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| (T)48: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCCAGCGCTGTAGTCTC | TCTGGTTTACTGGCCGTGAC | Start: 558 |
| Tm | 60.037 | 59.966 | End: 832 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
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| Sequence | GAGTCGATTTGCCCTGTCCA | GAGACTACAGCGCTGGGTTT | Start: 411 |
| Tm | 60.036 | 60.037 | End: 577 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
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| Sequence | GTCACGGCCAGTAAACCAGA | GAGCAGGTTTAGAGGCAGGG | Start: 813 |
| Tm | 59.966 | 60.107 | End: 927 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
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| Sequence | CCCTGCCTCTAAACCTGCTC | ATGCAATGGTCTGTGCTGGA | Start: 908 |
| Tm | 60.107 | 59.961 | End: 1151 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
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| Sequence | CATTGGGTGACGCGTTCTTG | TGGACAGGGCAAATCGACTC | Start: 286 |
| Tm | 60.11 | 60.036 | End: 430 |
| Size | 20 | 20 | 145 |
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