ssr_primer
Primer Details for TRINITY_DN63971_c1_g1_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCGAACTGTGCGAAAACA | AACACCACCCTTGAGCTGAC | Start: 55 |
| Tm | 59.973 | 60.179 | End: 333 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | ACTGCGAACTGTGCGAAAAC | AACACCACCCTTGAGCTGAC | Start: 54 |
| Tm | 59.972 | 60.179 | End: 333 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
|---|
| Sequence | GTCAGCTCAAGGGTGGTGTT | TCTGATCCCTGGAGGCAAGA | Start: 314 |
| Tm | 60.179 | 59.957 | End: 457 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | GTCAGCTCAAGGGTGGTGTT | TCTCTGATCCCTGGAGGCAA | Start: 314 |
| Tm | 60.179 | 59.957 | End: 459 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | TTGCCTCCAGGGATCAGAGA | AGGAACCACAAACGACCAGG | Start: 440 |
| Tm | 59.957 | 60.179 | End: 644 |
| Size | 20 | 20 | 205 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGCGAACTGTGCGAAAACA | AACACCACCCTTGAGCTGAC | Start: 55 |
| Tm | 59.973 | 60.179 | End: 333 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | ACTGCGAACTGTGCGAAAAC | AACACCACCCTTGAGCTGAC | Start: 54 |
| Tm | 59.972 | 60.179 | End: 333 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
|---|
| Sequence | GTCAGCTCAAGGGTGGTGTT | TCTGATCCCTGGAGGCAAGA | Start: 314 |
| Tm | 60.179 | 59.957 | End: 457 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | GTCAGCTCAAGGGTGGTGTT | TCTCTGATCCCTGGAGGCAA | Start: 314 |
| Tm | 60.179 | 59.957 | End: 459 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | TTGCCTCCAGGGATCAGAGA | AGGAACCACAAACGACCAGG | Start: 440 |
| Tm | 59.957 | 60.179 | End: 644 |
| Size | 20 | 20 | 205 |
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