ssr_primer
Primer Details for TRINITY_DN64059_c2_g6_i2 |
|---|
| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATCGACCAGCCAACCGAAC | GAAGGAAGTTTGGCCGCATG | Start: 473 |
| Tm | 60.109 | 60.109 | End: 687 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | ACTGGCCCTGAAAACGTGAT | GTTCGGTTGGCTGGTCGATA | Start: 351 |
| Tm | 59.89 | 60.109 | End: 492 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | GCCCTGAAAACGTGATTGGG | GTTCGGTTGGCTGGTCGATA | Start: 355 |
| Tm | 59.756 | 60.109 | End: 492 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | GGCCCTGAAAACGTGATTGG | GTTCGGTTGGCTGGTCGATA | Start: 354 |
| Tm | 59.756 | 60.109 | End: 492 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TATCGACCAGCCAACCGAAC | GGCTCCAAGGTTTCGTCACT | Start: 473 |
| Tm | 60.109 | 60.25 | End: 668 |
| Size | 20 | 20 | 196 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATCGACCAGCCAACCGAAC | GAAGGAAGTTTGGCCGCATG | Start: 473 |
| Tm | 60.109 | 60.109 | End: 687 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | ACTGGCCCTGAAAACGTGAT | GTTCGGTTGGCTGGTCGATA | Start: 351 |
| Tm | 59.89 | 60.109 | End: 492 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | GCCCTGAAAACGTGATTGGG | GTTCGGTTGGCTGGTCGATA | Start: 355 |
| Tm | 59.756 | 60.109 | End: 492 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | GGCCCTGAAAACGTGATTGG | GTTCGGTTGGCTGGTCGATA | Start: 354 |
| Tm | 59.756 | 60.109 | End: 492 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TATCGACCAGCCAACCGAAC | GGCTCCAAGGTTTCGTCACT | Start: 473 |
| Tm | 60.109 | 60.25 | End: 668 |
| Size | 20 | 20 | 196 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATCGACCAGCCAACCGAAC | GAAGGAAGTTTGGCCGCATG | Start: 473 |
| Tm | 60.109 | 60.109 | End: 687 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | ACTGGCCCTGAAAACGTGAT | GTTCGGTTGGCTGGTCGATA | Start: 351 |
| Tm | 59.89 | 60.109 | End: 492 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | GCCCTGAAAACGTGATTGGG | GTTCGGTTGGCTGGTCGATA | Start: 355 |
| Tm | 59.756 | 60.109 | End: 492 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | GGCCCTGAAAACGTGATTGG | GTTCGGTTGGCTGGTCGATA | Start: 354 |
| Tm | 59.756 | 60.109 | End: 492 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TATCGACCAGCCAACCGAAC | GGCTCCAAGGTTTCGTCACT | Start: 473 |
| Tm | 60.109 | 60.25 | End: 668 |
| Size | 20 | 20 | 196 |
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