ssr_primer
Primer Details for TRINITY_DN64143_c2_g2_i2 |
|---|
| (G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCACGCCAGTAGGAACA | GGATGGTTCGGCCTTGAGAA | Start: 1468 |
| Tm | 59.966 | 60.035 | End: 1623 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | TGTTCCACGCCAGTAGGAAC | GGATGGTTCGGCCTTGAGAA | Start: 1467 |
| Tm | 59.966 | 60.035 | End: 1623 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | ACCTGCAACGACCATGACTT | TCCCACGCTAGTTTGACCAC | Start: 926 |
| Tm | 59.891 | 59.966 | End: 1089 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | CAATGGCAGCAGTGTGACAC | CTACCCCTGATACGTGTGCC | Start: 200 |
| Tm | 60.04 | 59.896 | End: 439 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | AAGCTAGCATCCGGTCCAAC | GTGTCACACTGCTGCCATTG | Start: 32 |
| Tm | 60.108 | 60.04 | End: 219 |
| Size | 20 | 20 | 188 |
(A)10c(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCACGCCAGTAGGAACA | GGATGGTTCGGCCTTGAGAA | Start: 1468 |
| Tm | 59.966 | 60.035 | End: 1623 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | TGTTCCACGCCAGTAGGAAC | GGATGGTTCGGCCTTGAGAA | Start: 1467 |
| Tm | 59.966 | 60.035 | End: 1623 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | ACCTGCAACGACCATGACTT | TCCCACGCTAGTTTGACCAC | Start: 926 |
| Tm | 59.891 | 59.966 | End: 1089 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | CAATGGCAGCAGTGTGACAC | CTACCCCTGATACGTGTGCC | Start: 200 |
| Tm | 60.04 | 59.896 | End: 439 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | AAGCTAGCATCCGGTCCAAC | GTGTCACACTGCTGCCATTG | Start: 32 |
| Tm | 60.108 | 60.04 | End: 219 |
| Size | 20 | 20 | 188 |
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