ssr_primer
Primer Details for TRINITY_DN64178_c6_g2_i2 |
|---|
| (T)28: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGCGGGTCGATTGTTGAA | TGGGGCTCTGTGTAAACGAC | Start: 396 |
| Tm | 59.966 | 59.966 | End: 546 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | TCAGCGGGTCGATTGTTGAA | GCTCTGTGTAAACGACCCGA | Start: 396 |
| Tm | 59.966 | 60.039 | End: 542 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | TCAGCGGGTCGATTGTTGAA | TCGTTTATGCCTCGGAGTCG | Start: 396 |
| Tm | 59.966 | 59.9 | End: 668 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | GTCGTTTACACAGAGCCCCA | TCGTTTATGCCTCGGAGTCG | Start: 527 |
| Tm | 59.966 | 59.9 | End: 668 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | TCAGCGGGTCGATTGTTGAA | GAGGTGCAACGATCGGAGAT | Start: 396 |
| Tm | 59.966 | 59.897 | End: 565 |
| Size | 20 | 20 | 170 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGCGGGTCGATTGTTGAA | TGGGGCTCTGTGTAAACGAC | Start: 396 |
| Tm | 59.966 | 59.966 | End: 546 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | TCAGCGGGTCGATTGTTGAA | GCTCTGTGTAAACGACCCGA | Start: 396 |
| Tm | 59.966 | 60.039 | End: 542 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | TCAGCGGGTCGATTGTTGAA | TCGTTTATGCCTCGGAGTCG | Start: 396 |
| Tm | 59.966 | 59.9 | End: 668 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | GTCGTTTACACAGAGCCCCA | TCGTTTATGCCTCGGAGTCG | Start: 527 |
| Tm | 59.966 | 59.9 | End: 668 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | TCAGCGGGTCGATTGTTGAA | GAGGTGCAACGATCGGAGAT | Start: 396 |
| Tm | 59.966 | 59.897 | End: 565 |
| Size | 20 | 20 | 170 |
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