ssr_primer
Primer Details for TRINITY_DN64207_c5_g2_i2 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTGTCTGTCATGGTGGC | CAACTCCGCAACTCAGCAAC | Start: 856 |
| Tm | 59.965 | 60.041 | End: 1102 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TCCCTCAGACCACTCGGATT | CGGTACTGCGAACGTTGAAC | Start: 366 |
| Tm | 59.959 | 59.84 | End: 491 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | TCCCTCAGACCACTCGGATT | TCTCAGCGTAACGGTACTGC | Start: 366 |
| Tm | 59.959 | 59.83 | End: 502 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | AGCGTACATTTCCTCCGAGC | AATCCGAGTGGTCTGAGGGA | Start: 226 |
| Tm | 60.179 | 59.959 | End: 385 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | ATTATGTTGGCGCCGACTGA | GCTCGGAGGAAATGTACGCT | Start: 15 |
| Tm | 60.108 | 60.179 | End: 245 |
| Size | 20 | 20 | 231 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTGTCTGTCATGGTGGC | CAACTCCGCAACTCAGCAAC | Start: 856 |
| Tm | 59.965 | 60.041 | End: 1102 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TCCCTCAGACCACTCGGATT | CGGTACTGCGAACGTTGAAC | Start: 366 |
| Tm | 59.959 | 59.84 | End: 491 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | TCCCTCAGACCACTCGGATT | TCTCAGCGTAACGGTACTGC | Start: 366 |
| Tm | 59.959 | 59.83 | End: 502 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | AGCGTACATTTCCTCCGAGC | AATCCGAGTGGTCTGAGGGA | Start: 226 |
| Tm | 60.179 | 59.959 | End: 385 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | ATTATGTTGGCGCCGACTGA | GCTCGGAGGAAATGTACGCT | Start: 15 |
| Tm | 60.108 | 60.179 | End: 245 |
| Size | 20 | 20 | 231 |
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