ssr_primer
Primer Details for TRINITY_DN64217_c8_g1_i2 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGTGCGGCTATCAGATTC | TTTCTCATGGGGAAAGGGGC | Start: 170 |
| Tm | 59.968 | 59.959 | End: 298 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | TCGGTGCGGCTATCAGATTC | GGATAGCGACGTACGACCAG | Start: 170 |
| Tm | 59.968 | 60.041 | End: 373 |
| Size | 20 | 20 | 204 |
| Primer 3 details |
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| Sequence | CTGGTCGTACGTCGCTATCC | AGGATTCACGGGCGAACATT | Start: 354 |
| Tm | 60.041 | 60.035 | End: 613 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
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| Sequence | AGTTTTCGGTGCGGCTATCA | TTTCTCATGGGGAAAGGGGC | Start: 165 |
| Tm | 60.037 | 59.959 | End: 298 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | TCAGTTTTCGGTGCGGCTAT | TTTCTCATGGGGAAAGGGGC | Start: 163 |
| Tm | 60.037 | 59.959 | End: 298 |
| Size | 20 | 20 | 136 |
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