ssr_primer
Primer Details for TRINITY_DN64323_c6_g1_i2 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCATGAGAACGTCGGTGAA | TTCACAAAGGAGTCGGCCTC | Start: 737 |
| Tm | 60.038 | 59.965 | End: 954 |
| Size | 20 | 20 | 218 |
| Primer 2 details |
|---|
| Sequence | CCCAAGAGAACGTCGGTGAA | AAGGGACGGGTCATCTGAGA | Start: 859 |
| Tm | 59.967 | 59.959 | End: 1020 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | TCTCAGATGACCCGTCCCTT | AAGACTGGGCGATGACTTGG | Start: 1001 |
| Tm | 59.959 | 60.036 | End: 1254 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | TCTCAGATGACCCGTCCCTT | CCAAGACTGGGCGATGACTT | Start: 1001 |
| Tm | 59.959 | 60.036 | End: 1256 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | CCCAAGAGAACGTCGGTGAA | GACGGGTCATCTGAGAGCAG | Start: 859 |
| Tm | 59.967 | 59.898 | End: 1016 |
| Size | 20 | 20 | 158 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCATGAGAACGTCGGTGAA | TTCACAAAGGAGTCGGCCTC | Start: 737 |
| Tm | 60.038 | 59.965 | End: 954 |
| Size | 20 | 20 | 218 |
| Primer 2 details |
|---|
| Sequence | CCCAAGAGAACGTCGGTGAA | AAGGGACGGGTCATCTGAGA | Start: 859 |
| Tm | 59.967 | 59.959 | End: 1020 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | TCTCAGATGACCCGTCCCTT | AAGACTGGGCGATGACTTGG | Start: 1001 |
| Tm | 59.959 | 60.036 | End: 1254 |
| Size | 20 | 20 | 254 |
| Primer 4 details |
|---|
| Sequence | TCTCAGATGACCCGTCCCTT | CCAAGACTGGGCGATGACTT | Start: 1001 |
| Tm | 59.959 | 60.036 | End: 1256 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | CCCAAGAGAACGTCGGTGAA | GACGGGTCATCTGAGAGCAG | Start: 859 |
| Tm | 59.967 | 59.898 | End: 1016 |
| Size | 20 | 20 | 158 |
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