ssr_primer
Primer Details for TRINITY_DN64458_c4_g4_i1 |
|---|
| (A)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATAAACGGTCAAGCGGCT | TTAAGCGTCCCCAAGTCGAC | Start: 2 |
| Tm | 59.751 | 60.038 | End: 102 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | GTCGACTTGGGGACGCTTAA | TGTAAGTGCGGGATGGGAAG | Start: 83 |
| Tm | 60.038 | 59.748 | End: 353 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
|---|
| Sequence | GTCGACTTGGGGACGCTTAA | CTGTAAGTGCGGGATGGGAA | Start: 83 |
| Tm | 60.038 | 59.748 | End: 354 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | GTCGACTTGGGGACGCTTAA | GCGCCGCTTGCTACATAAAT | Start: 83 |
| Tm | 60.038 | 59.692 | End: 228 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | GTCGACTTGGGGACGCTTAA | GACAAGCTGTAAGTGCGGGA | Start: 83 |
| Tm | 60.038 | 60.32 | End: 360 |
| Size | 20 | 20 | 278 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATAAACGGTCAAGCGGCT | TTAAGCGTCCCCAAGTCGAC | Start: 2 |
| Tm | 59.751 | 60.038 | End: 102 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | GTCGACTTGGGGACGCTTAA | TGTAAGTGCGGGATGGGAAG | Start: 83 |
| Tm | 60.038 | 59.748 | End: 353 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
|---|
| Sequence | GTCGACTTGGGGACGCTTAA | CTGTAAGTGCGGGATGGGAA | Start: 83 |
| Tm | 60.038 | 59.748 | End: 354 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | GTCGACTTGGGGACGCTTAA | GCGCCGCTTGCTACATAAAT | Start: 83 |
| Tm | 60.038 | 59.692 | End: 228 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | GTCGACTTGGGGACGCTTAA | GACAAGCTGTAAGTGCGGGA | Start: 83 |
| Tm | 60.038 | 60.32 | End: 360 |
| Size | 20 | 20 | 278 |
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