ssr_primer
Primer Details for TRINITY_DN64974_c5_g8_i1 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGTTTCAAGTCACGTTT | GTGGAGGGGAAATCAGCACA | Start: 204 |
| Tm | 59.973 | 59.962 | End: 391 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | CGGCGTTTCAAGTCACGTTT | GCACAAATTGCCGGGAGATC | Start: 204 |
| Tm | 59.973 | 59.899 | End: 376 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | CGGCGTTTCAAGTCACGTTT | ACCCGATGCGGTGTAAAGAG | Start: 204 |
| Tm | 59.973 | 60.108 | End: 444 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | ATCGGCCAATTTGACGCATG | GTGGAGGGGAAATCAGCACA | Start: 259 |
| Tm | 59.899 | 59.962 | End: 391 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | CGGCGTTTCAAGTCACGTTT | CCCGATGCGGTGTAAAGAGA | Start: 204 |
| Tm | 59.973 | 59.825 | End: 443 |
| Size | 20 | 20 | 240 |
(CT)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCGTTTCAAGTCACGTTT | GTGGAGGGGAAATCAGCACA | Start: 204 |
| Tm | 59.973 | 59.962 | End: 391 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | CGGCGTTTCAAGTCACGTTT | GCACAAATTGCCGGGAGATC | Start: 204 |
| Tm | 59.973 | 59.899 | End: 376 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | CGGCGTTTCAAGTCACGTTT | ACCCGATGCGGTGTAAAGAG | Start: 204 |
| Tm | 59.973 | 60.108 | End: 444 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | ATCGGCCAATTTGACGCATG | GTGGAGGGGAAATCAGCACA | Start: 259 |
| Tm | 59.899 | 59.962 | End: 391 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | CGGCGTTTCAAGTCACGTTT | CCCGATGCGGTGTAAAGAGA | Start: 204 |
| Tm | 59.973 | 59.825 | End: 443 |
| Size | 20 | 20 | 240 |
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