ssr_primer
Primer Details for TRINITY_DN64992_c2_g1_i2 |
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| (T)11aaaaaaaaatagataatattttttttaaag(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCTGACTGACGGTACTTT | CGCAGCCATCCTTATTGCAC | Start: 431 |
| Tm | 60.039 | 59.97 | End: 543 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
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| Sequence | GCAAGGAAAACCCGAAACCC | AAAGTACCGTCAGTCAGCCG | Start: 240 |
| Tm | 59.967 | 60.039 | End: 450 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
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| Sequence | GATCGATCAGATGGCAGCGA | GGGTTTCGGGTTTTCCTTGC | Start: 96 |
| Tm | 60.039 | 59.967 | End: 259 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
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| Sequence | TCTTCGCAAGGAAAACCCGA | AAAGTACCGTCAGTCAGCCG | Start: 235 |
| Tm | 59.893 | 60.039 | End: 450 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
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| Sequence | GATCGATCAGATGGCAGCGA | TCGGGTTTTCCTTGCGAAGA | Start: 96 |
| Tm | 60.039 | 59.893 | End: 254 |
| Size | 20 | 20 | 159 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCTGACTGACGGTACTTT | CGCAGCCATCCTTATTGCAC | Start: 431 |
| Tm | 60.039 | 59.97 | End: 543 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | GCAAGGAAAACCCGAAACCC | AAAGTACCGTCAGTCAGCCG | Start: 240 |
| Tm | 59.967 | 60.039 | End: 450 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | GATCGATCAGATGGCAGCGA | GGGTTTCGGGTTTTCCTTGC | Start: 96 |
| Tm | 60.039 | 59.967 | End: 259 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
|---|
| Sequence | TCTTCGCAAGGAAAACCCGA | AAAGTACCGTCAGTCAGCCG | Start: 235 |
| Tm | 59.893 | 60.039 | End: 450 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | GATCGATCAGATGGCAGCGA | TCGGGTTTTCCTTGCGAAGA | Start: 96 |
| Tm | 60.039 | 59.893 | End: 254 |
| Size | 20 | 20 | 159 |
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