ssr_primer
Primer Details for TRINITY_DN65063_c1_g2_i3 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCGTGAAGGAGCTTGGTG | CATCCACTCTGACTCTCCGC | Start: 1176 |
| Tm | 59.966 | 59.898 | End: 1308 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
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| Sequence | TGCAGAATGAGCAGGCTTGA | TTCGCCACAACATCTGGTGA | Start: 216 |
| Tm | 59.963 | 59.892 | End: 440 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
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| Sequence | TGCAGAATGAGCAGGCTTGA | AACATCTGGTGAGCGAGCTC | Start: 216 |
| Tm | 59.963 | 60.108 | End: 432 |
| Size | 20 | 20 | 217 |
| Primer 4 details |
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| Sequence | TGCAGAATGAGCAGGCTTGA | GACGTTCGCCACAACATCTG | Start: 216 |
| Tm | 59.963 | 59.836 | End: 444 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
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| Sequence | TGCAGAATGAGCAGGCTTGA | CTGACGTTCGCCACAACATC | Start: 216 |
| Tm | 59.963 | 59.836 | End: 446 |
| Size | 20 | 20 | 231 |
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