Primer Details for TRINITY_DN65125_c2_g1_i1 |
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| (A)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGAAAAGCGAGAACAAATAGAA | TCGTCTACGTTTTTCTACTTCTTTTCT | Start: 16 |
| Tm | 57.27 | 59.082 | End: 175 |
| Size | 25 | 27 | 160 |
| Primer 2 details |
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| Sequence | AAGAAGAAAAGCGAGAACAAATAGA | TCGTCTACGTTTTTCTACTTCTTTTCT | Start: 15 |
| Tm | 57.27 | 59.082 | End: 175 |
| Size | 25 | 27 | 161 |
| Primer 3 details |
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| Sequence | AGAAGAAAAGCGAGAACAAATAGAA | TCGTCTACGTTTTTCTACTTCTTTT | Start: 16 |
| Tm | 57.27 | 57.008 | End: 175 |
| Size | 25 | 25 | 160 |
| Primer 4 details |
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| Sequence | AGAAGAAAAGCGAGAACAAATAGAA | TTCGTCTACGTTTTTCTACTTCTTT | Start: 16 |
| Tm | 57.27 | 57.008 | End: 176 |
| Size | 25 | 25 | 161 |
| Primer 5 details |
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| Sequence | AAGAAGAAAAGCGAGAACAAATAGA | TCGTCTACGTTTTTCTACTTCTTTT | Start: 15 |
| Tm | 57.27 | 57.008 | End: 175 |
| Size | 25 | 25 | 161 |
