Primer Details for TRINITY_DN65187_c2_g2_i2 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTATGTGGAGTCAGTCGCG | AAAGCATCAGTTTGTAAGGAAGGT | Start: 89 |
| Tm | 59.868 | 58.86 | End: 188 |
| Size | 21 | 24 | 100 |
| Primer 2 details | |||
| Sequence | TCTATGTGGAGTCAGTCGCG | AAAAGCATCAGTTTGTAAGGAAGGT | Start: 90 |
| Tm | 59.267 | 59.402 | End: 189 |
| Size | 20 | 25 | 100 |
| Primer 3 details | |||
| Sequence | TTCTATGTGGAGTCAGTCGCG | AAAAGCATCAGTTTGTAAGGAAGGT | Start: 89 |
| Tm | 59.868 | 59.402 | End: 189 |
| Size | 21 | 25 | 101 |
| Primer 4 details | |||
| Sequence | TCTTCTATGTGGAGTCAGTCGC | AGCATCAGTTTGTAAGGAAGGT | Start: 87 |
| Tm | 59.576 | 57.627 | End: 186 |
| Size | 22 | 22 | 100 |
| Primer 5 details | |||
| Sequence | TCTATGTGGAGTCAGTCGCG | AAAAGCATCAGTTTGTAAGGAAGG | Start: 90 |
| Tm | 59.267 | 57.911 | End: 189 |
| Size | 20 | 24 | 100 |
