ssr_primer
Primer Details for TRINITY_DN65203_c5_g1_i5 |
|---|
| (A)10(ATAAATAAATAAATAAATAAAAAAAATGAAAAAAATTCATTGGAATTATCAAATATTGGCAGTCGAGTTCATCGGGAGGACTCCGAGCTTGATTCCACGAAAACTTTCGCGAGAAACGAAACGAGTCGCGACAACAGACAAACGAAGACGGAGGGCGGTAGCGAATCAATCGCTTAATTTATCAGTCTACCTCATTTGTTTCCGCGATCCGCGCTCCCCCCGAGAAGAAACCCCGAAACGAGACGGGAAATAATACACAGTCGGAATTTATTTCTTTGAGCGCGCGGTCGAAGCTAAATTGCTGGTTGCATCCCATCCAGCTCCCGCCCCACCCTCCACGAGACTCGACGATGTCGAACGAGAGTGAAAAATCAACGGACGTCCCGCCCGATCGACTTGCCGCTCGTTCGTAATCCTCCCTTCTGCCGTGTTAAGGAAAAACGCCGTAGGAACACTCGCT)(AAAT)4: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCGAGAAGAAACCCCGAAA | CATCGTCGAGTCTCGTGGAG | Start: 919 |
| Tm | 59.964 | 59.973 | End: 1053 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | TGCGGATGTGACACTCGAAA | CTGTTCTCAAGGGGAAGCGT | Start: 75 |
| Tm | 59.967 | 59.965 | End: 175 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
|---|
| Sequence | TTGCGGATGTGACACTCGAA | CTGTTCTCAAGGGGAAGCGT | Start: 74 |
| Tm | 59.967 | 59.965 | End: 175 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
|---|
| Sequence | CTGTTTGGGTTCGGCACATG | AGTCATGGACAATGGCCAGG | Start: 468 |
| Tm | 60.039 | 60.034 | End: 591 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | GAGAAACGAAACGAGTCGCG | CATCGTCGAGTCTCGTGGAG | Start: 811 |
| Tm | 59.911 | 59.973 | End: 1053 |
| Size | 20 | 20 | 243 |
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