ssr_primer
Primer Details for TRINITY_DN65230_c7_g2_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAGTCAAAACCGAACGGA | CAGCGCGACAAGTGTGAAAA | Start: 190 |
| Tm | 59.897 | 59.973 | End: 352 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | TTTTCACACTTGTCGCGCTG | GGCCAGCCCTAATCTCGTTT | Start: 333 |
| Tm | 59.973 | 60.107 | End: 492 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | TTTTCACACTTGTCGCGCTG | TGGTGCATGAGGTGAAGCAA | Start: 333 |
| Tm | 59.973 | 60.179 | End: 519 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | AAACGAGATTAGGGCTGGCC | CGAAGGTCCCAGCCCTAATC | Start: 473 |
| Tm | 60.107 | 59.893 | End: 692 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | GACTTCTGACACGCGTACGA | GGCCAGCCCTAATCTCGTTT | Start: 256 |
| Tm | 60.111 | 60.107 | End: 492 |
| Size | 20 | 20 | 237 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAGTCAAAACCGAACGGA | CAGCGCGACAAGTGTGAAAA | Start: 190 |
| Tm | 59.897 | 59.973 | End: 352 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | TTTTCACACTTGTCGCGCTG | GGCCAGCCCTAATCTCGTTT | Start: 333 |
| Tm | 59.973 | 60.107 | End: 492 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | TTTTCACACTTGTCGCGCTG | TGGTGCATGAGGTGAAGCAA | Start: 333 |
| Tm | 59.973 | 60.179 | End: 519 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | AAACGAGATTAGGGCTGGCC | CGAAGGTCCCAGCCCTAATC | Start: 473 |
| Tm | 60.107 | 59.893 | End: 692 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | GACTTCTGACACGCGTACGA | GGCCAGCCCTAATCTCGTTT | Start: 256 |
| Tm | 60.111 | 60.107 | End: 492 |
| Size | 20 | 20 | 237 |
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