ssr_primer
Primer Details for TRINITY_DN65331_c5_g2_i1 |
|---|
| (G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTACAAGACACACGTGGGT | GGAGGGGGATTCGAACAGTG | Start: 746 |
| Tm | 59.895 | 60.108 | End: 973 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | GGTACAAGACACACGTGGGT | GTGGAGGGGGATTCGAACAG | Start: 746 |
| Tm | 59.895 | 60.108 | End: 975 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | AGTCCCGTTGGTCAGCAATT | ACCCACGTGTGTCTTGTACC | Start: 506 |
| Tm | 59.89 | 59.895 | End: 765 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | TGGGAGGGAACGGAGTGTAT | TTACGTTACGCTCGGCTTGA | Start: 199 |
| Tm | 59.958 | 59.759 | End: 407 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ATGGGAGGGAACGGAGTGTA | TTACGTTACGCTCGGCTTGA | Start: 198 |
| Tm | 59.958 | 59.759 | End: 407 |
| Size | 20 | 20 | 210 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTACAAGACACACGTGGGT | GGAGGGGGATTCGAACAGTG | Start: 746 |
| Tm | 59.895 | 60.108 | End: 973 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | GGTACAAGACACACGTGGGT | GTGGAGGGGGATTCGAACAG | Start: 746 |
| Tm | 59.895 | 60.108 | End: 975 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
|---|
| Sequence | AGTCCCGTTGGTCAGCAATT | ACCCACGTGTGTCTTGTACC | Start: 506 |
| Tm | 59.89 | 59.895 | End: 765 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | TGGGAGGGAACGGAGTGTAT | TTACGTTACGCTCGGCTTGA | Start: 199 |
| Tm | 59.958 | 59.759 | End: 407 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ATGGGAGGGAACGGAGTGTA | TTACGTTACGCTCGGCTTGA | Start: 198 |
| Tm | 59.958 | 59.759 | End: 407 |
| Size | 20 | 20 | 210 |
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