ssr_primer
Primer Details for TRINITY_DN65353_c3_g2_i2 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCTGCCACCAGAGTAAAT | GCCGATTGGAAGGTGAGGAA | Start: 77 |
| Tm | 60.034 | 60.035 | End: 264 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
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| Sequence | TTCCTCACCTTCCAATCGGC | GGGCCACGTTGAGAAGAGAA | Start: 245 |
| Tm | 60.035 | 59.965 | End: 421 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
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| Sequence | TTCCTCACCTTCCAATCGGC | ACTAACAGGGCCACGTTGAG | Start: 245 |
| Tm | 60.035 | 59.965 | End: 428 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
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| Sequence | GCCCTGCCACCAGAGTAAAT | CTTCAGTGACAGTGGCCGAT | Start: 77 |
| Tm | 60.034 | 60.037 | End: 356 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
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| Sequence | TTCCTCACCTTCCAATCGGC | CTTCAGTGACAGTGGCCGAT | Start: 245 |
| Tm | 60.035 | 60.037 | End: 356 |
| Size | 20 | 20 | 112 |
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