ssr_primer
Primer Details for TRINITY_DN65374_c2_g3_i2 |
|---|
| (TCT)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGAACGGATCCGAGTCTG | CTATCCTGCAACGCGTTTCG | Start: 78 |
| Tm | 59.972 | 59.975 | End: 231 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | CGAAACGCGTTGCAGGATAG | CTCCGTATCCGAGTGCACTC | Start: 212 |
| Tm | 59.975 | 59.97 | End: 490 |
| Size | 20 | 20 | 279 |
| Primer 3 details |
|---|
| Sequence | GCAGGACGGGTTTACATCCA | ATCGGTTTTCCTTTCGGCCT | Start: 523 |
| Tm | 60.036 | 59.962 | End: 749 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | GCAGGACGGGTTTACATCCA | CGGTTTTCCTTTCGGCCTTG | Start: 523 |
| Tm | 60.036 | 60.04 | End: 747 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | CGAAACGCGTTGCAGGATAG | TACGTGGTTTTTGCCCGGTA | Start: 212 |
| Tm | 59.975 | 59.893 | End: 385 |
| Size | 20 | 20 | 174 |
(G)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGAACGGATCCGAGTCTG | CTATCCTGCAACGCGTTTCG | Start: 78 |
| Tm | 59.972 | 59.975 | End: 231 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | CGAAACGCGTTGCAGGATAG | CTCCGTATCCGAGTGCACTC | Start: 212 |
| Tm | 59.975 | 59.97 | End: 490 |
| Size | 20 | 20 | 279 |
| Primer 3 details |
|---|
| Sequence | GCAGGACGGGTTTACATCCA | ATCGGTTTTCCTTTCGGCCT | Start: 523 |
| Tm | 60.036 | 59.962 | End: 749 |
| Size | 20 | 20 | 227 |
| Primer 4 details |
|---|
| Sequence | GCAGGACGGGTTTACATCCA | CGGTTTTCCTTTCGGCCTTG | Start: 523 |
| Tm | 60.036 | 60.04 | End: 747 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | CGAAACGCGTTGCAGGATAG | TACGTGGTTTTTGCCCGGTA | Start: 212 |
| Tm | 59.975 | 59.893 | End: 385 |
| Size | 20 | 20 | 174 |
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