ssr_primer
Primer Details for TRINITY_DN65479_c2_g1_i1 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATTCGGCGCCTCTTGTCTC | CCCAGCTTGAGAACAGAGCA | Start: 405 |
| Tm | 59.898 | 59.964 | End: 534 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
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| Sequence | TATTCGGCGCCTCTTGTCTC | TGCCCAGCTTGAGAACAGAG | Start: 405 |
| Tm | 59.898 | 59.964 | End: 536 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
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| Sequence | CTCTGTTCTCAAGCTGGGCA | ACTGCTGTGTTCCGTGGAAT | Start: 517 |
| Tm | 59.964 | 59.891 | End: 742 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
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| Sequence | TGCTCTGTTCTCAAGCTGGG | ACTGCTGTGTTCCGTGGAAT | Start: 515 |
| Tm | 59.964 | 59.891 | End: 742 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
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| Sequence | CTCTGTTCTCAAGCTGGGCA | TCTACTTTCTGCGCCTTCCG | Start: 517 |
| Tm | 59.964 | 60.109 | End: 703 |
| Size | 20 | 20 | 187 |
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