ssr_primer
Primer Details for TRINITY_DN65549_c8_g1_i2 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAGTTCAGACGTGTGCTC | CGCTAGGTTCAGCCTCAACA | Start: 5 |
| Tm | 59.968 | 60.037 | End: 183 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | CTGGAGTTCAGACGTGTGCT | CGCTAGGTTCAGCCTCAACA | Start: 4 |
| Tm | 59.967 | 60.037 | End: 183 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
|---|
| Sequence | GGTAGAAGGGAACGAGTGCC | ACGGTACAAATGCTCGCTCA | Start: 1254 |
| Tm | 60.108 | 60.038 | End: 1436 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
|---|
| Sequence | CAGACGTGTGCTCTTCCGAT | CGCTAGGTTCAGCCTCAACA | Start: 12 |
| Tm | 60.109 | 60.037 | End: 183 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | ACGTGTGCTCTTCCGATCTC | CGCTAGGTTCAGCCTCAACA | Start: 15 |
| Tm | 59.829 | 60.037 | End: 183 |
| Size | 20 | 20 | 169 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAGTTCAGACGTGTGCTC | CGCTAGGTTCAGCCTCAACA | Start: 5 |
| Tm | 59.968 | 60.037 | End: 183 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | CTGGAGTTCAGACGTGTGCT | CGCTAGGTTCAGCCTCAACA | Start: 4 |
| Tm | 59.967 | 60.037 | End: 183 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
|---|
| Sequence | GGTAGAAGGGAACGAGTGCC | ACGGTACAAATGCTCGCTCA | Start: 1254 |
| Tm | 60.108 | 60.038 | End: 1436 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
|---|
| Sequence | CAGACGTGTGCTCTTCCGAT | CGCTAGGTTCAGCCTCAACA | Start: 12 |
| Tm | 60.109 | 60.037 | End: 183 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | ACGTGTGCTCTTCCGATCTC | CGCTAGGTTCAGCCTCAACA | Start: 15 |
| Tm | 59.829 | 60.037 | End: 183 |
| Size | 20 | 20 | 169 |
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