ssr_primer
Primer Details for TRINITY_DN65636_c5_g2_i2 |
|---|
| (G)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGTTCCAGGTCTGCGCAA | AAATTTCTGGGGGAGGCTCG | Start: 3 |
| Tm | 60.037 | 60.034 | End: 174 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | AGTTCCAGGTCTGCGCAATT | AAATTTCTGGGGGAGGCTCG | Start: 5 |
| Tm | 60.251 | 60.034 | End: 174 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
|---|
| Sequence | CTAGTTCCAGGTCTGCGCAA | ACCAAACACCTACCCATCGG | Start: 3 |
| Tm | 60.037 | 59.673 | End: 115 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
|---|
| Sequence | CGAGCCTCCCCCAGAAATTT | GATGAGCACCGTCGAGATGA | Start: 155 |
| Tm | 60.034 | 59.618 | End: 362 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | CCAGGTCTGCGCAATTTTCG | AAATTTCTGGGGGAGGCTCG | Start: 9 |
| Tm | 60.455 | 60.034 | End: 174 |
| Size | 20 | 20 | 166 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGTTCCAGGTCTGCGCAA | AAATTTCTGGGGGAGGCTCG | Start: 3 |
| Tm | 60.037 | 60.034 | End: 174 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | AGTTCCAGGTCTGCGCAATT | AAATTTCTGGGGGAGGCTCG | Start: 5 |
| Tm | 60.251 | 60.034 | End: 174 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
|---|
| Sequence | CTAGTTCCAGGTCTGCGCAA | ACCAAACACCTACCCATCGG | Start: 3 |
| Tm | 60.037 | 59.673 | End: 115 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
|---|
| Sequence | CGAGCCTCCCCCAGAAATTT | GATGAGCACCGTCGAGATGA | Start: 155 |
| Tm | 60.034 | 59.618 | End: 362 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | CCAGGTCTGCGCAATTTTCG | AAATTTCTGGGGGAGGCTCG | Start: 9 |
| Tm | 60.455 | 60.034 | End: 174 |
| Size | 20 | 20 | 166 |
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