ssr_primer
Primer Details for TRINITY_DN65868_c7_g3_i2 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAACGAAAACAGGCAGGTC | GGTGTCTCCAAGTCCGGAAA | Start: 115 |
| Tm | 59.689 | 59.605 | End: 364 |
| Size | 20 | 20 | 250 |
| Primer 2 details |
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| Sequence | CCAACGAAAACAGGCAGGTC | TTTTTGAGTGCGGTCCGGAA | Start: 115 |
| Tm | 59.689 | 60.464 | End: 334 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
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| Sequence | TTTTCAGTGCTTCCGGACCG | TGAGTGTTTGGGAGGGGGTA | Start: 305 |
| Tm | 60.883 | 60.104 | End: 406 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
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| Sequence | CGGACTTTTTCAGTGCTTCCG | GTTTGGGAGGGGGTAAGTGG | Start: 299 |
| Tm | 60.07 | 59.961 | End: 401 |
| Size | 21 | 20 | 103 |
| Primer 5 details |
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| Sequence | AACAGGCCAACGAAAACAGG | GGTGTCTCCAAGTCCGGAAA | Start: 109 |
| Tm | 59.256 | 59.605 | End: 364 |
| Size | 20 | 20 | 256 |
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