ssr_primer
Primer Details for TRINITY_DN65881_c1_g1_i1 |
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| (T)11atttttttttatttttttatatttttttattta(T)10atttattta(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTATCCTATCCGCCTGGGG | CACTGTGCTCCGTTGTAGGT | Start: 176 |
| Tm | 59.959 | 59.966 | End: 291 |
| Size | 20 | 20 | 116 |
| Primer 2 details |
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| Sequence | AGTTTTTCCTGGCTGTCCCC | CACTGTGCTCCGTTGTAGGT | Start: 35 |
| Tm | 60.179 | 59.966 | End: 291 |
| Size | 20 | 20 | 257 |
| Primer 3 details |
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| Sequence | ACCTACAACGGAGCACAGTG | GCCGCACAATGGATCAAGTG | Start: 272 |
| Tm | 59.966 | 60.179 | End: 518 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
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| Sequence | AGTTTTTCCTGGCTGTCCCC | CCCCAGGCGGATAGGATAGA | Start: 35 |
| Tm | 60.179 | 59.959 | End: 195 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
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| Sequence | TCTATCCTATCCGCCTGGGG | GACTCGATCCACTGTGCTCC | Start: 176 |
| Tm | 59.959 | 60.179 | End: 300 |
| Size | 20 | 20 | 125 |
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