ssr_primer
Primer Details for TRINITY_DN65888_c3_g1_i12 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCCCGGAAATTTATGCAG | AGACGAGTCGTAGGTGGTGA | Start: 623 |
| Tm | 59.969 | 59.964 | End: 854 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
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| Sequence | TCCAGAAAATTAGCGGGCGT | AAGGGAAAACAGGGTTGCCA | Start: 1845 |
| Tm | 60.036 | 60.032 | End: 1978 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | TGTCAACCATAGGCAAGCGT | AAGGGAAAACAGGGTTGCCA | Start: 1878 |
| Tm | 59.964 | 60.032 | End: 1978 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
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| Sequence | TGTGCCTCGGTATTCACCAC | AAGGGAAAACAGGGTTGCCA | Start: 1805 |
| Tm | 60.037 | 60.032 | End: 1978 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
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| Sequence | TCCAGAAAATTAGCGGGCGT | GTTGCCACCACACGTAGAGA | Start: 1845 |
| Tm | 60.036 | 59.967 | End: 1965 |
| Size | 20 | 20 | 121 |
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