ssr_primer
Primer Details for TRINITY_DN66170_c6_g1_i6 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTCTGTTGTGGAGGGGA | ACCACCTTACGCAGTTCTCG | Start: 663 |
| Tm | 60.105 | 60.039 | End: 792 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
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| Sequence | TCCTTCTGTTGTGGAGGGGA | CCACCTTACGCAGTTCTCGA | Start: 663 |
| Tm | 60.105 | 59.757 | End: 791 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
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| Sequence | TGCCTGTCTTGGGTTCCCTA | TTCGTTGCCGGAACTCAGTT | Start: 2351 |
| Tm | 60.475 | 60.179 | End: 2528 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
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| Sequence | TGCCTGTCTTGGGTTCCCTA | GCTGCTCGAATTGCCATCAC | Start: 2351 |
| Tm | 60.475 | 60.248 | End: 2478 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
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| Sequence | ATCAATCCGTCGATTCCGCC | TTCGTTGCCGGAACTCAGTT | Start: 2429 |
| Tm | 60.6 | 60.179 | End: 2528 |
| Size | 20 | 20 | 100 |
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