ssr_primer
Primer Details for TRINITY_DN66176_c1_g3_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCACTCATACCTCCGTCGC | TAGCCGTGTCAAACTTCCCC | Start: 350 |
| Tm | 59.97 | 59.964 | End: 582 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | CTCACTCATACCTCCGTCGC | TCGGAGGAGAATTTCTCGCG | Start: 350 |
| Tm | 59.97 | 59.899 | End: 512 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | TCCTCTTGACGCGATATGCC | TCGGAGGAGAATTTCTCGCG | Start: 285 |
| Tm | 59.968 | 59.899 | End: 512 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | TCCTCTTGACGCGATATGCC | GGTCGTGCGATGTTTTTCCC | Start: 285 |
| Tm | 59.968 | 60.11 | End: 394 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | GGGGAAGTTTGACACGGCTA | CCGGAAATGACCCCTCTTCC | Start: 563 |
| Tm | 59.964 | 60.107 | End: 686 |
| Size | 20 | 20 | 124 |
(C)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCACTCATACCTCCGTCGC | TAGCCGTGTCAAACTTCCCC | Start: 350 |
| Tm | 59.97 | 59.964 | End: 582 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | CTCACTCATACCTCCGTCGC | TCGGAGGAGAATTTCTCGCG | Start: 350 |
| Tm | 59.97 | 59.899 | End: 512 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | TCCTCTTGACGCGATATGCC | TCGGAGGAGAATTTCTCGCG | Start: 285 |
| Tm | 59.968 | 59.899 | End: 512 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | TCCTCTTGACGCGATATGCC | GGTCGTGCGATGTTTTTCCC | Start: 285 |
| Tm | 59.968 | 60.11 | End: 394 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | GGGGAAGTTTGACACGGCTA | CCGGAAATGACCCCTCTTCC | Start: 563 |
| Tm | 59.964 | 60.107 | End: 686 |
| Size | 20 | 20 | 124 |
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