ssr_primer
Primer Details for TRINITY_DN66388_c4_g1_i6 |
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| (AAAT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTGTGGCTCAACAAACTCG | GGAGATGGGAGGGTTAGGGT | Start: 657 |
| Tm | 60.042 | 60.03 | End: 769 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
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| Sequence | TTTCGCCCTGCAGTTTGTTG | AGGTCGTAGGGCTCAAGGAT | Start: 190 |
| Tm | 59.898 | 60.032 | End: 297 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
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| Sequence | CGCCCTGCAGTTTGTTGATC | AGGTCGTAGGGCTCAAGGAT | Start: 193 |
| Tm | 60.11 | 60.032 | End: 297 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
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| Sequence | TTTGGCTATTCCTTCCGCGT | CCGGACTTTCGAAACAAGCG | Start: 221 |
| Tm | 60.036 | 60.111 | End: 372 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
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| Sequence | TTTGGCTATTCCTTCCGCGT | ATGTTCTCACGACGGAGCTC | Start: 221 |
| Tm | 60.036 | 59.829 | End: 333 |
| Size | 20 | 20 | 113 |
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