Primer Details for TRINITY_DN66409_c3_g1_i1 |
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| (A)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAGCTACAAAATAGATTAGTGTCC | TTTTTGCGAGGGATTTGCGA | Start: 34 |
| Tm | 60.018 | 59.045 | End: 139 |
| Size | 26 | 20 | 106 |
| Primer 2 details |
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| Sequence | GCCAGCTACAAAATAGATTAGTGTCC | TTGCGAGGGATTTGCGATTT | Start: 34 |
| Tm | 60.018 | 58.827 | End: 136 |
| Size | 26 | 20 | 103 |
| Primer 3 details |
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| Sequence | GCCAGCTACAAAATAGATTAGTGTCC | TTTGCGAGGGATTTGCGATT | Start: 34 |
| Tm | 60.018 | 58.827 | End: 137 |
| Size | 26 | 20 | 104 |
| Primer 4 details |
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| Sequence | GCCAGCTACAAAATAGATTAGTGTCC | TTTTGCGAGGGATTTGCGAT | Start: 34 |
| Tm | 60.018 | 58.827 | End: 138 |
| Size | 26 | 20 | 105 |
| Primer 5 details |
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| Sequence | AGCTACAAAATAGATTAGTGTCCCCT | TTTTTGCGAGGGATTTGCGA | Start: 37 |
| Tm | 59.613 | 59.045 | End: 139 |
| Size | 26 | 20 | 103 |
