ssr_primer
Primer Details for TRINITY_DN66434_c2_g1_i1 |
|---|
| (C)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAAATTTATCAGGCCGCCG | GGTGGAGGGAGGAGAGGAAT | Start: 293 |
| Tm | 59.969 | 60.031 | End: 458 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | CCAAATTTATCAGGCCGCCG | AATACCCTCCCACTCAGGCT | Start: 293 |
| Tm | 59.969 | 59.955 | End: 517 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | CCAAATTTATCAGGCCGCCG | TTTTCCTGGTGGAGGGAGGA | Start: 293 |
| Tm | 59.969 | 60.104 | End: 465 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | ATTCCTCTCCTCCCTCCACC | CCACTTTGGACGACTGGTGA | Start: 439 |
| Tm | 60.031 | 59.894 | End: 616 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | CCAAATTTATCAGGCCGCCG | CCCACTCAGGCTGCTATCAG | Start: 293 |
| Tm | 59.969 | 59.894 | End: 509 |
| Size | 20 | 20 | 217 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAAATTTATCAGGCCGCCG | GGTGGAGGGAGGAGAGGAAT | Start: 293 |
| Tm | 59.969 | 60.031 | End: 458 |
| Size | 20 | 20 | 166 |
| Primer 2 details |
|---|
| Sequence | CCAAATTTATCAGGCCGCCG | AATACCCTCCCACTCAGGCT | Start: 293 |
| Tm | 59.969 | 59.955 | End: 517 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | CCAAATTTATCAGGCCGCCG | TTTTCCTGGTGGAGGGAGGA | Start: 293 |
| Tm | 59.969 | 60.104 | End: 465 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | ATTCCTCTCCTCCCTCCACC | CCACTTTGGACGACTGGTGA | Start: 439 |
| Tm | 60.031 | 59.894 | End: 616 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | CCAAATTTATCAGGCCGCCG | CCCACTCAGGCTGCTATCAG | Start: 293 |
| Tm | 59.969 | 59.894 | End: 509 |
| Size | 20 | 20 | 217 |
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