ssr_primer
Primer Details for TRINITY_DN66483_c2_g2_i2 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATTCGTGGCGATCATCC | ACGGCTGCAAGTGAACAAAC | Start: 307 |
| Tm | 59.966 | 59.899 | End: 577 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
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| Sequence | AGGATTCGTGGCGATCATCC | GCTTACGGCTGCAAGTGAAC | Start: 307 |
| Tm | 59.966 | 60.11 | End: 581 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
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| Sequence | CAGAGGATTCGTGGCGATCA | ACGGCTGCAAGTGAACAAAC | Start: 304 |
| Tm | 59.897 | 59.899 | End: 577 |
| Size | 20 | 20 | 274 |
| Primer 4 details |
|---|
| Sequence | ACAGAGGATTCGTGGCGATC | ACGGCTGCAAGTGAACAAAC | Start: 303 |
| Tm | 59.897 | 59.899 | End: 577 |
| Size | 20 | 20 | 275 |
| Primer 5 details |
|---|
| Sequence | CAGAGGATTCGTGGCGATCA | GCTTACGGCTGCAAGTGAAC | Start: 304 |
| Tm | 59.897 | 60.11 | End: 581 |
| Size | 20 | 20 | 278 |
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