ssr_primer
Primer Details for TRINITY_DN66517_c5_g2_i2 |
|---|
| (T)32: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGAAAAGCTCCGGTATGT | TTCTTGTGCCGCATCAGAGT | Start: 665 |
| Tm | 60.108 | 59.965 | End: 835 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | TTTTGCAGATGGGGTGTCGA | TTCTTGTGCCGCATCAGAGT | Start: 722 |
| Tm | 59.89 | 59.965 | End: 835 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | CGAGTTTCGCCAACTTGGTG | TCGAACTGAGCCGATTTCCC | Start: 739 |
| Tm | 60.041 | 60.108 | End: 865 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | CGGGAAAAGCTCCGGTATGT | TCGAACTGAGCCGATTTCCC | Start: 665 |
| Tm | 60.108 | 60.108 | End: 865 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TTTTGCAGATGGGGTGTCGA | TCGAACTGAGCCGATTTCCC | Start: 722 |
| Tm | 59.89 | 60.108 | End: 865 |
| Size | 20 | 20 | 144 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGAAAAGCTCCGGTATGT | TTCTTGTGCCGCATCAGAGT | Start: 665 |
| Tm | 60.108 | 59.965 | End: 835 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
|---|
| Sequence | TTTTGCAGATGGGGTGTCGA | TTCTTGTGCCGCATCAGAGT | Start: 722 |
| Tm | 59.89 | 59.965 | End: 835 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | CGAGTTTCGCCAACTTGGTG | TCGAACTGAGCCGATTTCCC | Start: 739 |
| Tm | 60.041 | 60.108 | End: 865 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | CGGGAAAAGCTCCGGTATGT | TCGAACTGAGCCGATTTCCC | Start: 665 |
| Tm | 60.108 | 60.108 | End: 865 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TTTTGCAGATGGGGTGTCGA | TCGAACTGAGCCGATTTCCC | Start: 722 |
| Tm | 59.89 | 60.108 | End: 865 |
| Size | 20 | 20 | 144 |
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