ssr_primer
Primer Details for TRINITY_DN66533_c2_g2_i5 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTAAGGCGCGTGCGAAAAA | TGCGATCCGAATAAAGCCGA | Start: 332 |
| Tm | 59.971 | 59.897 | End: 604 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
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| Sequence | TTTAAGGCGCGTGCGAAAAA | GGAAAGGCCACTCGGGTATC | Start: 332 |
| Tm | 59.971 | 60.179 | End: 550 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
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| Sequence | TCGGCTTTATTCGGATCGCA | ACACTCCTCACCTGCAACAG | Start: 585 |
| Tm | 59.897 | 59.893 | End: 762 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
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| Sequence | AACTCAAGAGATGGGGCTGC | GGAAAGGCCACTCGGGTATC | Start: 283 |
| Tm | 60.035 | 60.179 | End: 550 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
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| Sequence | CGTGCGAAAAATGGGGAGAC | TGCGATCCGAATAAAGCCGA | Start: 341 |
| Tm | 59.832 | 59.897 | End: 604 |
| Size | 20 | 20 | 264 |
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