ssr_primer
Primer Details for TRINITY_DN66657_c0_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACCTTTTCGACAGGGTGGT | TCTGCGCGGGTATAATGTCC | Start: 514 |
| Tm | 59.892 | 59.967 | End: 756 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
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| Sequence | TGCTGGCTTTCGATTCGAGT | ACCACCCTGTCGAAAAGGTC | Start: 415 |
| Tm | 60.037 | 59.892 | End: 533 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
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| Sequence | GGGAATACAGCGTCGGTTCA | ACTCGAATCGAAAGCCAGCA | Start: 289 |
| Tm | 60.109 | 60.037 | End: 434 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
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| Sequence | CAAATGGAGTGTTCGCGACG | TCTGCGCGGGTATAATGTCC | Start: 617 |
| Tm | 60.179 | 59.967 | End: 756 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
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| Sequence | CGACAAATGGAGTGTTCGCG | TCTGCGCGGGTATAATGTCC | Start: 614 |
| Tm | 60.179 | 59.967 | End: 756 |
| Size | 20 | 20 | 143 |
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