ssr_primer
Primer Details for TRINITY_DN66974_c2_g4_i3 |
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| (C)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGACAGAATGCGCCTCAGT | GGATCGGAAACTATGGCGGT | Start: 317 |
| Tm | 59.965 | 59.894 | End: 484 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
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| Sequence | TTGACAGAATGCGCCTCAGT | GGTTAGGCATCGCAAAGCAC | Start: 317 |
| Tm | 59.965 | 60.179 | End: 467 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
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| Sequence | ATTGCCGACTAACGTCCTCG | GGATCGGAAACTATGGCGGT | Start: 250 |
| Tm | 60.179 | 59.894 | End: 484 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
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| Sequence | TTGACAGAATGCGCCTCAGT | AGGCATCGCAAAGCACTAGA | Start: 317 |
| Tm | 59.965 | 59.75 | End: 463 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
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| Sequence | ATTGCCGACTAACGTCCTCG | GGTTAGGCATCGCAAAGCAC | Start: 250 |
| Tm | 60.179 | 60.179 | End: 467 |
| Size | 20 | 20 | 218 |
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