ssr_primer
Primer Details for TRINITY_DN67308_c8_g1_i2 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAACGTCGCCAAGAACTT | CTCCGCCATAGCCAGATCTG | Start: 1087 |
| Tm | 59.971 | 60.038 | End: 1312 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CAGATCTGGCTATGGCGGAG | CCACGTGAGCCTCTTCCTTT | Start: 1293 |
| Tm | 60.038 | 59.965 | End: 1565 |
| Size | 20 | 20 | 273 |
| Primer 3 details |
|---|
| Sequence | GCAAACGTCGCCAAGAACTT | CCACCAATCGGAGGAGCTAC | Start: 1087 |
| Tm | 59.971 | 59.895 | End: 1292 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | GCAAACGTCGCCAAGAACTT | AGCCAGATCTGCCACCAATC | Start: 1087 |
| Tm | 59.971 | 60.107 | End: 1303 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | GCAAACGTCGCCAAGAACTT | AAACCTTCCTCCGCCATAGC | Start: 1087 |
| Tm | 59.971 | 60.107 | End: 1320 |
| Size | 20 | 20 | 234 |
(T)10c(T)10gtaa(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAACGTCGCCAAGAACTT | CTCCGCCATAGCCAGATCTG | Start: 1087 |
| Tm | 59.971 | 60.038 | End: 1312 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CAGATCTGGCTATGGCGGAG | CCACGTGAGCCTCTTCCTTT | Start: 1293 |
| Tm | 60.038 | 59.965 | End: 1565 |
| Size | 20 | 20 | 273 |
| Primer 3 details |
|---|
| Sequence | GCAAACGTCGCCAAGAACTT | CCACCAATCGGAGGAGCTAC | Start: 1087 |
| Tm | 59.971 | 59.895 | End: 1292 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | GCAAACGTCGCCAAGAACTT | AGCCAGATCTGCCACCAATC | Start: 1087 |
| Tm | 59.971 | 60.107 | End: 1303 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | GCAAACGTCGCCAAGAACTT | AAACCTTCCTCCGCCATAGC | Start: 1087 |
| Tm | 59.971 | 60.107 | End: 1320 |
| Size | 20 | 20 | 234 |
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