ssr_primer
Primer Details for TRINITY_DN67908_c3_g2_i2 |
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| (A)23gggctccatt(C)11ttcatttcttttttgaaactttatgaaggtattaatctgcagttttcactttcaagttcattt(A)40: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTACCGCAGAGGCTGTTAC | GCCCAACAGGGTCACTAGAC | Start: 249 |
| Tm | 59.754 | 60.036 | End: 493 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
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| Sequence | CCCGCAATTGTCGTTCATGG | GCCATTGACAGCAAGCAGAG | Start: 770 |
| Tm | 60.179 | 59.829 | End: 919 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
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| Sequence | GAGGCTGTTACGGTAGCGAT | GCCCAACAGGGTCACTAGAC | Start: 258 |
| Tm | 59.615 | 60.036 | End: 493 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
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| Sequence | AAAAAGGGCTCCATTCCCCC | TGGTGCTTTGGCTCAGTTCT | Start: 73 |
| Tm | 60.253 | 59.817 | End: 227 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
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| Sequence | AAAAAGGGCTCCATTCCCCC | TCTTGGTGCTTTGGCTCAGT | Start: 73 |
| Tm | 60.253 | 59.817 | End: 230 |
| Size | 20 | 20 | 158 |
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