ssr_primer
Primer Details for TRINITY_DN68220_c3_g1_i4 |
|---|
| (C)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCCCAGTGCTCAATCGA | GGGCTACCAGACAAGGTACG | Start: 37 |
| Tm | 60.036 | 59.825 | End: 189 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | TAAGACCATAGTGCGCCTCG | GGGCTACCAGACAAGGTACG | Start: 8 |
| Tm | 59.616 | 59.825 | End: 189 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
|---|
| Sequence | CTGGATTCCAGCAGCACCTA | AGCGGCCATCAGAAATGGAA | Start: 678 |
| Tm | 59.457 | 60.034 | End: 837 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | CTGGATTCCAGCAGCACCTA | GAAGTTTCTCTCCCCGGAGC | Start: 678 |
| Tm | 59.457 | 60.108 | End: 871 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
|---|
| Sequence | CTGGATTCCAGCAGCACCTA | CAGTGTAGCGGCCATCAGAA | Start: 678 |
| Tm | 59.457 | 60.108 | End: 843 |
| Size | 20 | 20 | 166 |
(C)12(A)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCCCAGTGCTCAATCGA | GGGCTACCAGACAAGGTACG | Start: 37 |
| Tm | 60.036 | 59.825 | End: 189 |
| Size | 20 | 20 | 153 |
| Primer 2 details |
|---|
| Sequence | TAAGACCATAGTGCGCCTCG | GGGCTACCAGACAAGGTACG | Start: 8 |
| Tm | 59.616 | 59.825 | End: 189 |
| Size | 20 | 20 | 182 |
| Primer 3 details |
|---|
| Sequence | CTGGATTCCAGCAGCACCTA | AGCGGCCATCAGAAATGGAA | Start: 678 |
| Tm | 59.457 | 60.034 | End: 837 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | CTGGATTCCAGCAGCACCTA | GAAGTTTCTCTCCCCGGAGC | Start: 678 |
| Tm | 59.457 | 60.108 | End: 871 |
| Size | 20 | 20 | 194 |
| Primer 5 details |
|---|
| Sequence | CTGGATTCCAGCAGCACCTA | CAGTGTAGCGGCCATCAGAA | Start: 678 |
| Tm | 59.457 | 60.108 | End: 843 |
| Size | 20 | 20 | 166 |
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