ssr_primer
Primer Details for TRINITY_DN68237_c3_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTCCTGCGGTGTAAGTAG | AATCACCTTAAGCCAGCCCC | Start: 297 |
| Tm | 60.179 | 60.033 | End: 426 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
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| Sequence | AAGCTTTTTGAGTGGCCAGC | AATCACCTTAAGCCAGCCCC | Start: 189 |
| Tm | 59.61 | 60.033 | End: 426 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
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| Sequence | AAGCTTTTTGAGTGGCCAGC | ACCATCGAACTTCGGTCACC | Start: 189 |
| Tm | 59.61 | 60.038 | End: 389 |
| Size | 20 | 20 | 201 |
| Primer 4 details |
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| Sequence | GTGGTTATAGTGCTCGCCCT | AATCACCTTAAGCCAGCCCC | Start: 316 |
| Tm | 59.536 | 60.033 | End: 426 |
| Size | 20 | 20 | 111 |
| Primer 5 details |
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| Sequence | AGTGGTTATAGTGCTCGCCC | AATCACCTTAAGCCAGCCCC | Start: 315 |
| Tm | 59.536 | 60.033 | End: 426 |
| Size | 20 | 20 | 112 |
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