ssr_primer
Primer Details for TRINITY_DN68329_c2_g1_i1 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAATGATGTGTGTGCCCCA | CGGACCTCCTCCAGTTGAAC | Start: 86 |
| Tm | 59.888 | 60.037 | End: 327 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | GTCAATGATGTGTGTGCCCC | CGGACCTCCTCCAGTTGAAC | Start: 85 |
| Tm | 59.472 | 60.037 | End: 327 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | TCAATGATGTGTGTGCCCCA | ACGGACCTCCTCCAGTTGAA | Start: 86 |
| Tm | 59.888 | 60.471 | End: 328 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | TCAATGATGTGTGTGCCCCA | TTGACGGACCTCCTCCAGTT | Start: 86 |
| Tm | 59.888 | 60.471 | End: 331 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | GGGACGACGACTCTTGGAAT | CCAACTCGGATCACCTGTCC | Start: 288 |
| Tm | 59.469 | 60.108 | End: 403 |
| Size | 20 | 20 | 116 |
(G)21cggccc(G)12aacg(C)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAATGATGTGTGTGCCCCA | CGGACCTCCTCCAGTTGAAC | Start: 86 |
| Tm | 59.888 | 60.037 | End: 327 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | GTCAATGATGTGTGTGCCCC | CGGACCTCCTCCAGTTGAAC | Start: 85 |
| Tm | 59.472 | 60.037 | End: 327 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | TCAATGATGTGTGTGCCCCA | ACGGACCTCCTCCAGTTGAA | Start: 86 |
| Tm | 59.888 | 60.471 | End: 328 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | TCAATGATGTGTGTGCCCCA | TTGACGGACCTCCTCCAGTT | Start: 86 |
| Tm | 59.888 | 60.471 | End: 331 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | GGGACGACGACTCTTGGAAT | CCAACTCGGATCACCTGTCC | Start: 288 |
| Tm | 59.469 | 60.108 | End: 403 |
| Size | 20 | 20 | 116 |
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