ssr_primer
Primer Details for TRINITY_DN68353_c1_g5_i5 |
|---|
| (T)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCCTTGTCATCCAAATCC | CCACGAAGTACATCTCCCCG | Start: 1267 |
| Tm | 59.899 | 59.898 | End: 1377 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | GGTGCTCGAAACGGACCATA | GGATTTGGATGACAAGGCGC | Start: 1045 |
| Tm | 60.109 | 59.899 | End: 1286 |
| Size | 20 | 20 | 242 |
| Primer 3 details |
|---|
| Sequence | GCGCCTTGTCATCCAAATCC | CAGAAATCCGCTCCTCCACA | Start: 1267 |
| Tm | 59.899 | 59.75 | End: 1445 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | GCGCCTTGTCATCCAAATCC | AGAAATCCGCTCCTCCACAC | Start: 1267 |
| Tm | 59.899 | 59.749 | End: 1444 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | TCATCTGTGCCTAACGCGTT | TAAGCTGCATGACACCGTGA | Start: 180 |
| Tm | 60.038 | 59.68 | End: 444 |
| Size | 20 | 20 | 265 |
(T)21: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCCTTGTCATCCAAATCC | CCACGAAGTACATCTCCCCG | Start: 1267 |
| Tm | 59.899 | 59.898 | End: 1377 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | GGTGCTCGAAACGGACCATA | GGATTTGGATGACAAGGCGC | Start: 1045 |
| Tm | 60.109 | 59.899 | End: 1286 |
| Size | 20 | 20 | 242 |
| Primer 3 details |
|---|
| Sequence | GCGCCTTGTCATCCAAATCC | CAGAAATCCGCTCCTCCACA | Start: 1267 |
| Tm | 59.899 | 59.75 | End: 1445 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | GCGCCTTGTCATCCAAATCC | AGAAATCCGCTCCTCCACAC | Start: 1267 |
| Tm | 59.899 | 59.749 | End: 1444 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
|---|
| Sequence | TCATCTGTGCCTAACGCGTT | TAAGCTGCATGACACCGTGA | Start: 180 |
| Tm | 60.038 | 59.68 | End: 444 |
| Size | 20 | 20 | 265 |
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