ssr_primer
Primer Details for TRINITY_DN68506_c0_g2_i1 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCACAGTCAATGGACCCCC | CCCTTCCCCGTATGACATCG | Start: 134 |
| Tm | 60.035 | 59.966 | End: 378 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CGATGTCATACGGGGAAGGG | AAACTACCCTCTTTGGCCGG | Start: 359 |
| Tm | 59.966 | 59.962 | End: 619 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | CGATGTCATACGGGGAAGGG | CCGTTCAGCCGTCCAAAAAG | Start: 359 |
| Tm | 59.966 | 60.04 | End: 463 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | CTTTTTGGACGGCTGAACGG | AAACTACCCTCTTTGGCCGG | Start: 444 |
| Tm | 60.04 | 59.962 | End: 619 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CTCACAGTCAATGGACCCCC | ATTTGCCCCTTCCCCGTATG | Start: 134 |
| Tm | 60.035 | 60.106 | End: 384 |
| Size | 20 | 20 | 251 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCACAGTCAATGGACCCCC | CCCTTCCCCGTATGACATCG | Start: 134 |
| Tm | 60.035 | 59.966 | End: 378 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | CGATGTCATACGGGGAAGGG | AAACTACCCTCTTTGGCCGG | Start: 359 |
| Tm | 59.966 | 59.962 | End: 619 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | CGATGTCATACGGGGAAGGG | CCGTTCAGCCGTCCAAAAAG | Start: 359 |
| Tm | 59.966 | 60.04 | End: 463 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | CTTTTTGGACGGCTGAACGG | AAACTACCCTCTTTGGCCGG | Start: 444 |
| Tm | 60.04 | 59.962 | End: 619 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CTCACAGTCAATGGACCCCC | ATTTGCCCCTTCCCCGTATG | Start: 134 |
| Tm | 60.035 | 60.106 | End: 384 |
| Size | 20 | 20 | 251 |
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