ssr_primer
Primer Details for TRINITY_DN68713_c0_g2_i2 |
|---|
| (A)34: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTGTTTGCCCTGCATGAA | GCCGAGATACTCAGGCTCAC | Start: 2039 |
| Tm | 59.966 | 59.969 | End: 2265 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | ATGCTTGTTTGCCCTGCATG | GCCGAGATACTCAGGCTCAC | Start: 2037 |
| Tm | 60.037 | 59.969 | End: 2265 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GTCGGGCGTTAAAGTCTCCA | CCGACTAAGAGGATGGTCGC | Start: 5595 |
| Tm | 60.038 | 59.969 | End: 5716 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | GGCACAGTCAAGAGCTAGCA | TCGACTGACAGTTGAGGTGC | Start: 2741 |
| Tm | 60.037 | 59.968 | End: 2898 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | GGCACAGTCAAGAGCTAGCA | CGACTGACAGTTGAGGTGCT | Start: 2741 |
| Tm | 60.037 | 59.967 | End: 2897 |
| Size | 20 | 20 | 157 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTGTTTGCCCTGCATGAA | GCCGAGATACTCAGGCTCAC | Start: 2039 |
| Tm | 59.966 | 59.969 | End: 2265 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | ATGCTTGTTTGCCCTGCATG | GCCGAGATACTCAGGCTCAC | Start: 2037 |
| Tm | 60.037 | 59.969 | End: 2265 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GTCGGGCGTTAAAGTCTCCA | CCGACTAAGAGGATGGTCGC | Start: 5595 |
| Tm | 60.038 | 59.969 | End: 5716 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | GGCACAGTCAAGAGCTAGCA | TCGACTGACAGTTGAGGTGC | Start: 2741 |
| Tm | 60.037 | 59.968 | End: 2898 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | GGCACAGTCAAGAGCTAGCA | CGACTGACAGTTGAGGTGCT | Start: 2741 |
| Tm | 60.037 | 59.967 | End: 2897 |
| Size | 20 | 20 | 157 |
(T)10atttttatattatttttataatttttaccccgatcgataaaataacgaatggaggtctgagg(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTTGTTTGCCCTGCATGAA | GCCGAGATACTCAGGCTCAC | Start: 2039 |
| Tm | 59.966 | 59.969 | End: 2265 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
|---|
| Sequence | ATGCTTGTTTGCCCTGCATG | GCCGAGATACTCAGGCTCAC | Start: 2037 |
| Tm | 60.037 | 59.969 | End: 2265 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GTCGGGCGTTAAAGTCTCCA | CCGACTAAGAGGATGGTCGC | Start: 5595 |
| Tm | 60.038 | 59.969 | End: 5716 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | GGCACAGTCAAGAGCTAGCA | TCGACTGACAGTTGAGGTGC | Start: 2741 |
| Tm | 60.037 | 59.968 | End: 2898 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | GGCACAGTCAAGAGCTAGCA | CGACTGACAGTTGAGGTGCT | Start: 2741 |
| Tm | 60.037 | 59.967 | End: 2897 |
| Size | 20 | 20 | 157 |
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