Primer Details for TRINITY_DN69143_c6_g1_i1 | |||
|---|---|---|---|
| (T)10(A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGTACGTGCTAGAAAGTTTTT | ACATCACTAATAATGGAACCCGA | Start: 35 |
| Tm | 57.428 | 57.376 | End: 211 |
| Size | 23 | 23 | 177 |
| Primer 2 details | |||
| Sequence | CGAGTACGTGCTAGAAAGTTTTT | ACCTAACGAATCTCTTCTCCAAA | Start: 35 |
| Tm | 57.428 | 57.26 | End: 163 |
| Size | 23 | 23 | 129 |
| Primer 3 details | |||
| Sequence | TGTCCTCTCTAGTGAAAATACGAGT | ACATCACTAATAATGGAACCCGA | Start: 15 |
| Tm | 59.055 | 57.376 | End: 211 |
| Size | 25 | 23 | 197 |
| Primer 4 details | |||
| Sequence | TGTCCTCTCTAGTGAAAATACGAGT | ACCTAACGAATCTCTTCTCCAAA | Start: 15 |
| Tm | 59.055 | 57.26 | End: 163 |
| Size | 25 | 23 | 149 |
| Primer 5 details | |||
| Sequence | CGAGTACGTGCTAGAAAGTTTTT | ACATCACTAATAATGGAACCCGAT | Start: 35 |
| Tm | 57.428 | 57.812 | End: 211 |
| Size | 23 | 24 | 177 |
