ssr_primer
Primer Details for TRINITY_DN69201_c2_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTGGGGAAAAATACCGT | ACCAAGCTATTTTGCGGGGA | Start: 661 |
| Tm | 59.963 | 59.961 | End: 854 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCCCCGCAAAATAGCTTGGT | CCACCCTGGTTCGACTCAAA | Start: 835 |
| Tm | 59.961 | 59.892 | End: 963 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | TCTAATGCTGTCACGCTGGG | ACCAAGCTATTTTGCGGGGA | Start: 649 |
| Tm | 60.108 | 59.961 | End: 854 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | TCCCCGCAAAATAGCTTGGT | TTTTCCCACCCTGGTTCGAC | Start: 835 |
| Tm | 59.961 | 60.179 | End: 968 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | CGCTGGGGAAAAATACCGTG | ACCAAGCTATTTTGCGGGGA | Start: 662 |
| Tm | 59.55 | 59.961 | End: 854 |
| Size | 20 | 20 | 193 |
(T)15gctttccccgcaaaatagcttggtccaaagtttggcagaaaaaaatttacgaccagttgaattcaaaactactcctaactgctaa(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGCTGGGGAAAAATACCGT | ACCAAGCTATTTTGCGGGGA | Start: 661 |
| Tm | 59.963 | 59.961 | End: 854 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCCCCGCAAAATAGCTTGGT | CCACCCTGGTTCGACTCAAA | Start: 835 |
| Tm | 59.961 | 59.892 | End: 963 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | TCTAATGCTGTCACGCTGGG | ACCAAGCTATTTTGCGGGGA | Start: 649 |
| Tm | 60.108 | 59.961 | End: 854 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | TCCCCGCAAAATAGCTTGGT | TTTTCCCACCCTGGTTCGAC | Start: 835 |
| Tm | 59.961 | 60.179 | End: 968 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | CGCTGGGGAAAAATACCGTG | ACCAAGCTATTTTGCGGGGA | Start: 662 |
| Tm | 59.55 | 59.961 | End: 854 |
| Size | 20 | 20 | 193 |
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